Increased global energy consumption demands the use of more energy resources, aggravating environmental issues. This study focused on analyzing biogas production from a mixture of cow dung, water hyacinth, and food waste and checking the efficiency of the biogas. The efficiency of biogas production was tested using two alternative settings in the study. The first setup employs Eichhornia crassipes that have been NaOH-treated and mixed with co-digestion substrates such as cow manure and food waste which have been stored at room temperature for 32 days. The second setup contains five different types of substrates such as L1-cow dung, L2- cow dung: water hyacinth, L3-cow dung: food waste, L4-cow dung: water hyacinth: food waste, and L5-water hyacinth. The properties of the Eichhornia crassipes were studied on several biogas substrates, such as pH, temperature, COD, TOC, and NPK tests, as well as total biogas output and methane percentage. The results of the comparison analysis show that the substrate L4 has a high level of NPK (4.7 %) and a higher amount of COD (137600 mg/l). These characteristics enhance the gas yield and methane percentage (85 %). Overall, the water hyacinth mixed with cow dung and food waste exceeded the other four substrates. The total yield of biogas from the first setup was 8.5 litres, the flammability was tested on the 28th day, and the blue flame was obtained. Water hyacinth was removed from aquatic areas and used as an alternative energy source, hence being environmentally friendly.