Document Type : Research Article


Department of Mechanical Engineering, Faculty of Engineering, Alzahra University, Tehran, Iran.



Microbial Fuel Cells (MFCs) represent an environmentally-friendly approach to generating electricity, but the need to study variation parameters to find improvement conditions has been an important challenge for decades. In this study, a single-chamber MFC was designed to investigate the key parameters such as the concentration and type of bacteria, chamber temperature, electrode spacing, and substrate rotation speed that affected the performance of MFCs. Therefore, two types of bacteria, Shewanella oneidensis ( and Escherichia coli (E. coli), were compared as microorganisms. Then, the function of MFC was investigated under the following condition: three temperatures (30 ℃, 45℃, and 60℃), three bacterial concentrations (0.5% (v/v) (4.5 mg/ml), 1% (v/v) (9mg/ml), and 1.5% (v/v) (13.5mg/ml)), electrode distances (2 cm, 3 cm, 4cm), and substrate speeds (100 rpm, 150 rpm, 200 rpm). Ultimately, ( bacteria, a chamber temperature of 45 ℃, a bacterial concentration of 1% (v/v) (9mg/ml), a cathode-anode spacing of 3 cm, and a rotation speed of 150 rpm proved to be the most efficient parameter settings for the constructed microbial fuel cell. The maximum voltage and highest power density were 486.9 mV and 9.73 mW/ , respectively, with a resistance of 7500 ohms. These results are meaningful for determining and improving important parameters in an MFC device.


Main Subjects

  1. Bharat Mishra, Sanjay Kumar Awasthi, Raj Kumar Rajak, “A Review on Electrical Behavior of Different Substrates, Electrodes and Membranes in Microbial Fuel Cell”, International Journal of Energy and Power Engineering, Vol. 11, No. 9, (2017), (
  2. Clare Y. Cui, “The Effect of Anode Surface Structures on Microbial Fuel Cells”, Thesis Department of Mechanical Engineering at The Ohio State University, P. 88, (2016), (
  3. Keshavarz, M., Mohebbi-Kalhori, D. and Yousefi, V., “Multi-response optimization of tubular microbial fuel cells using response surface methodology (RSM)”, Journal of Renewable Energy and Environment (JREE), Vol. 9, No. 2, (2022), 49-58. (
  4. Pandey, Garima., “Biomass based bio-electro fuel cells based on carbon electrodes: an alternative source of renewable energy”, SN Applied Sciences, (2019), (
  5. Yujin Cao, Hui Mu, Wei Liu, Rubing Zhang, Jing Guo, Mo Xian, and Huizhou Liu, “Electricians in the anode of microbial fuel cells: pure cultures versus mixed communities”, Microbe Cell Fact, 18, P. 39, (2019), (
  6. Xavier Alexis Walter⁎, Jiseon You, Jonathan Winfield, Ugnius Bajarunas, John Greenman, Ioannis A. Ieropoulos, “From the lab to the field: Self-stratifying microbial fuel cells stacks directly powering lights”, Applied Energy, Vol 277, (2020), (
  7. Arvin Taghizadeh Tabrizi, Hossein Aghajani, Farhad Farhang Laleh, “Review on the Materials for Hydrogen Adsorption & Storage”, jrenew, (2019), (In Farsi), (
  8. KeChrist Obileke, Helen Onyeaka, Edson L Meyer, Nwabunwanne Nwokolo, “Microbial fuel cells, a renewable energy technology for bio-electricity generation”, Electrochemistry Communications, Vol. 125, (2021), (
  9. J. Salar-García, I. Ieropoulos, “Optimisation of the internal structure of ceramic membranes for electricity production in urine-fed microbial fuel cells”, Journal of Power Sources, Vol. 451, (2020), (
  10. Yilkal Dessie, Sisay Tadesse, Rajalakshmanan Eswaramoorthy, “Review on manganese oxide-based biocatalyst in microbial fuel cell: Nanocomposite approach”, Materials Science for Energy Technologies, Vol. 3, P. 136-149, (2020), (
  11. Iwona Gajda, John Greenman, Ioannis Ieropoulos, “Microbial Fuel Cell stack performance enhancement through carbon veil anode modification with activated carbon powder”, Applied Energy, Vol. 262, (2020), (
  12. Sara Mateo, Pablo Cañizares, Manuel Andrés Rodrigo, Francisco Jesus Fernandez-Morales, “driving force behind electrochemical performance of microbial fuel cells fed with different substrates”, Chemosphere, Vol. 207, (2018), (https://DOI: 10.1016/j.chemosphere.2018.05.100)
  13. Abhishek J. Chhazed, Manisha V. Makwana, Neeraj K. Chavda, “Microbial Fuel Cell Functioning, Developments and Applications”, International journal of scientific & technology research, Vol. 8, (2019), (
  14. Manon Oliot, Benjamin Erable, Marie-Line De Solan, Alain Bergel., “Increasing the temperature is a relevant strategy to form microbial anodes intended to work at room temperature”, Electrochemical Acta, Elsevier, P.134-142, (2017), ff10.1016/j.electacta.2017.10.110ff. ffhal-01877656f
  15. Jumma, S. and N. Patil. “Microbial Fuel Cell: Design and Operation.”, Journal of Microbiology and Biotechnology, (2016), (
  16. Adriana Páez, Andrea Lache-Muñoz, Sergio Medina, Julieta Zapata4, “Electric power production in a microbial fuel cell using Escherichia coli and Pseudomonas aeruginosa, synthetic wastewater as substrate, carbon cloth and graphite as electrodes, and methylene blue as mediator”, Laboratory scale, vol. 10, (2019), (https://DOI: 10.24850/j-tyca-2019-06-11)
  17. Nguyen, Dang-Trang & Tamura, Teppei & Tobe, Ryuta & Mihara, Hisaaki & Taguchi, Kozo., “Microbial fuel cell performance improvement based on FliC-deficient E. coli strain.”, Energy Reports, Vol. 6, P. 763-767, (2020), (https://DOI:10.1016/j.egyr.2020.11.133)
  18. Xiaoxue Mei, Defeng Xing, Yang Yang, Qian Liu, Huihui Zhou, Changhong Guo, Nanqi Ren, “Adaptation of microbial community of the anode biofilm in microbial fuel cells to temperature”, Bioelectrochemistry, (2017), (https://doi: 10.1016/ j. bioelechem.2017.04.005)
  19. Ezziat L, Elabed A, Ibnsouda S and El Abed S, “Challenges of Microbial Fuel Cell Architecture on Heavy Metal Recovery and Removal from Wastewater.”, Front Energy, (2019), (https://doi: 10.3389/fenrg.2019.00001)
  20. Oluwatosin Obata, Maria J. Salar-Garcia, John Greenman, Halil Kurt, Kartik Chandran, Ioannis Ieropoulos, “Development of efficient electroactive biofilm in urine-fed microbial fuel cell cascades for bioelectricity generation”, Journal of Environmental Management, Vol. 258, (2020), (
  21. Tan SM, Ong SA, Ho LN, Wong YS, Thung WE, Teoh TP., “The reaction of wastewater treatment and power generation of single chamber microbial fuel cell against substrate concentration and anode distributions.”, Journal Environment Health Sci Eng, Vol. 18, P. 793-807, (2020), (https://doi: 10.1007/s40201-020-00504-w. PMID: 33312603; PMCID: PMC7721755)
  22. László Koók, Nándor Nemestóthy, Katalin Bélafi-Bakó, Péter Bakonyi, “Treatment of dark fermentative H2 production effluents by microbial fuel cells: A tutorial review on promising operational strategies and practices”, International Journal of Hydrogen Energy, Vol. 46, P. 5556-5569, (2021), (
  23. Shengnan Li, Shih-Hsin Ho, Tao Hua, Qixing Zhou, Fengxiang Li, Jingchun Tang, “Sustainable biochar as an electrocatalysts for the oxygen reduction reaction in microbial fuel cells”, Green Energy & Environment, Vol. 6, P. 644-659, (2021), (
  24. Imologie M Simeon, Katharina Herkendell, Deepak Pant, Ruth Freitag, “Electrochemical evaluation of different polymer binders for the production of carbon-modified stainless-steel electrodes for sustainable power generation using a soil microbial fuel cell”, Chemical Engineering Journal Advances, Vol. 10, (2022), (
  25. Kiaeenajad A, Moqtaderi H, Mahmoodi N, Maerufi S. “Design and Construction of a Microbial Fuel Cell for Electricity Generation from Municipal Wastewater Using Industrial Vinasse as Substrate.”, Modares Mechanical Engineering., P. 2403-2412, (2020), (In Farsi). (
  26. J. Salar-García, X.A. Walter, J. Gurauskis, A. de Ramón Fernández, I. Ieropoulos, “Effect of iron oxide content and microstructural porosity on the performance of ceramic membranes as microbial fuel cell separators”, Electrochemica Acta, Vol. 367, (2021), (
  27. John Greenman, Buddhi Arjuna Mendis, Iwona Gajda, Ioannis A. Ieropoulos, “Microbial fuel cell compared to a chemostat”, Chemosphere, Vol. 296, (2022), (
  28. Simeon M. Imologie, Raji O. A, Agidi Gbabo, Okoro‐Shekwaga C.A, “Performance of a Single Chamber Soil Microbial Fuel Cell at Varied External Resistances for Electric Power Generation”, Journal of Renewable Energy and Environment, Vol 3, P. 53-58, (2016), (
  29. Flimban, S.G.A.; Kim, T.; Ismail, I.M.I.; Oh, S. Overview of Microbial Fuel Cell (MFC) Recent Advancement from Fundamentals to Applications: MFC Designs, Major Elements, andScalability”, Preprints, (2018), (https://doi:10.20944/preprints201810. 0763.v1.)
  30. Angelaalincy MJ, Navanietha Krishnaraj R, Shakambari G, Ashokkumar B, Kathiresan S and Varalakshmi P, “Biofilm Engineering Approaches for Improving the Performance of Microbial Fuel Cells and Bio Electrochemical Systems.”, Front Energy, (2018), (https://doi: 10.3389/fenrg.2018.00063)
  31. G Bhargavi, V Venu, S Renganathan, “Microbial fuel cells: recent developments in design and materials”, IOP Conf. Series: Material Science and Engineering, (2018), (https://doi:10.1088/1757-899X/330/1/012034)
  32. Lee CY, Huang YN., “The effects of electrode spacing on the performance of microbial fuel cells under different substrate concentrations.”, Water Sci Technol., (2013), (https://doi: 10.2166/wst.2013.446. PMID: 24225104.)
  33. Lalitha Priya R, Ramachandran T, Suneesh P V, “Fabrication and characterization of high power dual chamber E. coli microbial fuel cell”, Materials Science and Engineering, (2016), (https://doi:10.1088/1757-899X/149/1/012215)
  34. Şen-Doğan B, Okan M, Afşar-Erkal N, Özgür E, Zorlu Ö, Külah H., “Enhancement of the Start-Up Time for Microliter-Scale Microbial Fuel Cells (µMFCs) via the Surface Modification of Gold Electrodes.”, Micromachines (Basel)., P. 703., (2020), (https://doi: 10.3390/mi11070703. PMID: 32708083; PMCID: PMC7407754.)
  35. S R Juliastuti1, R Darmawan1, A Ayuningtyas1 and N Ellyza1., “The utilization of Escherichia coli and Shewanella oneidensis for microbial fuel cell”, The 3rd International Conference on Chemical Engineering Sciences and Applications, 334, P. 20–21, (2017), (
  36. Wang, V., Sivakumar, K., Yang, L. et al., “Metabolite-enabled mutualistic interaction between Shewanella oneidensis and Escherichia coli in a co-culture using an electrode as electron acceptor.”, Science Reports, (2015). (https://DOI:10.1038/srep11222)
  37. Singh, Amandeep & Krishnamurthy, Balaji. (2019). “Parametric modeling of microbial fuel cells”, Journal of Electrochemical Science and Engineering., Vol. 9(4), P. 311-323, (2019), (
  38. Feng, Yujie, Lee, He, Wang, Xin, Liu, Yaolan, “Electricity Generation in Microbial Fuel Cells at Different Temperature and Isolation of Electrogenic Bacteria”, Asia-Pacific Power and Energy Engineering Conference, (2019), (
  39. Marzieh Aghababaie, Mehrdad Farhadian, Azam Jeihanipour & David Biria. “Effective factors on the performance of microbial fuel cells in wastewater treatment”, Environmental Technology Reviews, 4:1, P. 71-89, (2015), (
  40. Ren, H., Jiang, C. & Chae, J., “Effect of temperature on a miniaturized microbial fuel cell (MFC).”, Micro and Nano Syst, Vol. 5, P. 13, (2017), (
  41. Siddharth Gadkaria, b, Jean-Marie Fontmorinc, Eileen Yuc, Jhuma Sadhukhana, “Influence of temperature and other system parameters on microbial fuel cell performance: Numerical and experimental investigation”, Chemical Engineering Journal, P. 388 (2020), (
  42. Al-Asheh, Sameer & Al-Assaf, Yousef & Aidan, A, “Single-Chamber Microbial Fuel Cells’ Behavior at Different Operational Scenarios”, Energies, 13, P. 5458, (2020), (https://doi:10.3390/en13205458)
  43. Foad marashi, Seyed kamran & Kariminia, Hamid-Reza., “Performance of a single chamber microbial fuel cell at different organic loads and pH values using purified terephthalic acid wastewater”, Journal of Environmental Health Science and Engineering., Vol. 13, (2015), (
  44. Morio Miyahara Meidensha, Atsushi Kouzuma, Kazuya Watanabe, “Effects of NaCl concentration on anode microbes in microbial fuel cells”, AMB Expr, (2015), (
  45. Hongjun Ni, Kaixuan Wang, Shuaishuai Lv, Xingxing Wang, Lu Zhuo and Jiaqiao Zhang, “Effects of Concentration Variations on the Performance and Microbial Community in Microbial Fuel Cell Using Swine Wastewater”, Energies, (2020), (https://doi:10.3390/su10072446)
  46. Xiaoying Kong, Gaixiu Yang and Yongming Sun, “Performance Investigation of Batch Mode Microbial Fuel Cells Fed with High Concentration of Glucose”, Journal of Scientific and Technical Research, (2018), (https://DOI: 10.26717/BJSTR.2018.03.000864)
  47. Ardiyan Harimawan, Hary Devianto, Rd. Habib R. M. T. Al-Aziz, Dian Shofinita, Tjandra Setiadi, “influence of Electrode Distance on Electrical Energy Production of Microbial Fuel Cell using Tapioca Wastewater”, Journal of Engineering and Technological Sciences, 50(6), P. 841-855, (2018), (https://DOI: 10.5614/j.eng.technol.sci.2018.50.6.7)
  48. Nancy González-Gamboa, Xochitl Domínguez-Benetton, Daniella Pacheco-Catalán, Sathish Kumar-Kamaraj, David Valdés-Lozano, Jorge Domínguez-Maldonadoand Liliana Alzate-Gaviria, “Effect of Operating Parameters on the Performance Evaluation of Benthic Microbial Fuel Cells Using Sediments from the Bay of Campeche”, Journal of Sustainability, (2018), (https://doi:10.3390/su10072446)
  49. Yuan Pana, b, Tong Zhua, Zhen Heb, “Energy advantage of anode electrode rotation over anolyte recirculation for operating a tubular microbial fuel cell”, Electrochemistry Communications, (2019), (
  50. Yiyang Liua, Xiaoyan Sunb, Di Yina, Lankun Caia and Lehua Zhang, “Suspended anode-type microbial fuel cells for enhanced electricity generation”, RSC Adv., Vol. 10, P. 9868-9877, (2020), (https://DOI: 10.1039/c9ra08288c)
  51. Marwa S. Hamed, Hasan Sh. Majdi, and Basim O. Hasan, “Effect of Electrode Material and Hydrodynamics on the Produced Current in Double Chamber Microbial Fuel Cells”, ACS Omega, Vol. 5, P. 18, (2020), (https://DOI: 10.1021/acsomega.9b04451)