Document Type : Research Article
Authors
1 Department of Chemistry, E.M.U. Magusa, Turkey
2 Department of Chemical Engineering, Tarbiat Modares University, Tehran, Iran
Abstract
Regarding some reported antimicrobial properties of tannins; Zymomonas mobilis was used to obtain ethanol from tannin-reduced carob pod extract (TR-CPE). Culture of 50 mL volume containing 7.5 g sugar at pH 5.5 and 0.03 g bacterial inoculums with shaking at 80 rpm was used. Using response surface methodology (RSM), the maximum ethanol concentration of 5.34 % w/v (higher than that reported earlier, 4.01% w/v) was obtained at the optimized addition levels of yeast extract, peptone, and fermentation time; 0.13 g, 0.62 g, and 43.78 h, respectively. Carob pods extract (CPE) containing 62.23 gl-1 sugar was treated with 3 gl-1 gelatin to decrease its tannin content by 57.87 %. Sugar loss was not observed during gelatin treatment. The results revealed that there was no significant difference in ethanol production, yield, and productivity between TR-CPE and non-TR-CPE. In conclusion, tannin showed no inhibiting effect to maximum ethanol production by Zymomonas mobilis.
Keywords
- Morton J.F. Fruits of warm climates, Miami FL, 1987, 65-69.
- FAO. FAOSTAT. Food and Agric Organization of the United Nations, 2011.
- Battle I., Tous J. Carob tree (Ceratoniasiliqua L.). IPGRI, Rome, Italy, 1997, 23-64.
- Yousif A.K., Alghzawi H.M. Processing and characterization of carob powder. Food Chem, 2000, 69, 283–287.
- Vaheed H., Shojaosadati S.A., Galip H. Evaluation and optimization of ethanol production from carob pod extract by Zymomonas mobilis using response surface methodology. J Ind Microbiol Biotech, 2011, 38, 101–111.
- Biner B., Gubbuk H., Karhan M., Aksu M., Pekmezi M. Sugar profiles of the pods of cultivated and wild types of carob bean (Ceratoniasiliqua) in Turkey. Food Chem, 2007, 100, 1453-1455.
- Balat M., Balat H. Recent trends in global production and utilization of bio-ethanol fuel. Appl Energ, 2009, 86, 2273-2282.
- Roukas T. Ethanol-production from carob pods by Saccharomy cescerevisae. Food Biotechnol, 1993, 7(2), 159-176.
- Turhan I., Bialka K., Demirchi A., Karhan M. Ethanol productionfrom carob extract by using Saccharomy cescerevisiae. Bioresour Technol, 2010, 101, 5290–5296.
- Mazaheri D., Shojaosadati S. A., Mousav S.M., Hejazi P., Saharkhiz S. Bioethanol production from carob pods by solid-state fermentation with Zymomonas mobilis. Appl Energ, 2012, 99, 372-378.
- Nachtomi E., Alumot E. Tannin and polyphenols in carob pods (Ceratoniasiliqua). J Sci Food Agr, 1963, 14, 464-468.
- Bate-smith E.C., Swain T. Flavonoid compounds, in Florkin M., Mason H S. (Ed.), Comp Biochem, AP, New-York, 1962, 3, 75-80.
- Henis Y., Tagari H., Volcani R. Effect of water extracts of Carob pods, tannic acid and their derivatives on the morphology and growth of microorganisms. Appl Microbiol, 1964, 12(3), 204-209.
- Yoshida T., Mori K., Hatano T., Okumura T., Uehara I., Komagoe K., Fujita Y., Okuda T. Studies on inhibition mechanism of autoxidation by tannins and flavonoids. V. radical-scavenging effect of tannins and related polyphenols on 1, 1-diphenyl-2-picrylhydrazyl radical. Chem Pharm Bull, 1989, 37, 1919-1921.
- Manal H., Ahmad M., Ghaleb A. Carob fruit as source of carbon and energy for production of Saccharomyces cerevisiae. Jordan J Biol Sci, 2012, 5(3), 209 – 214.
- Shuping Y., Xiaorong Wu., Faubion Jon., Scott R., Bean Cai., Liming Shi., Yong-Cheng Xiuzhi S., Sun Wang D. Ethanol-production performance of ozone-treated tannin grain sorghum flour. Cereal Chem, 2012, 89(1), 30-37.
- Ying Lin. Gu Bi, Li Kai1, Dai Dao-fang. Effect of tannin on alcoholic fermentation of cassava. Guangxi Tropical Agric, 2010, (5).
- Scalhert A. Antimicrobial properties of tannins. Phytochem, 1991, 30, 3875.
- Ringland C., Eschenbruch R. Gelatin for juice and wine fining. Food Technol in New Zealand, Aug, 1983.
- Maruthai K., Thangavelu V., Kanagasabai M. Statistical screening of medium components on ethanol production from cashew apple juice using Saccharomyces diasticus. Int J Chem & Bio Eng, 2012, 6, 108-111.
- Roukas T. Continuous ethanol production from non-sterilized carob pod extract by immobilized Saccharomyces cerevisiae on mineral kissiris using a two-reactor system. Appl Biochem Biotechnol, 1996, 59, 299–307.
- Miller G.L. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem, 1959, 31, 426–428.
- Roukas T. Kinetic of ethanol-production from carob pods extract by immobilized Saccharomyces cerevisiae cells. Appl Biochem Biotechnol, 1994, 44, 49–64.
- Caputi Jr.A., Ueda M., Brown T. Spectrophotometric determination of ethanol in wine. Am J Enol Viticult, 1968, 19, 160–165.
- Oi-Wah L., Shiu-Fai L., Hsiao-Lan H. Spectrometric determination of tannins in tea and beer samples with iron (III) and 1, 10-Phenantroline as reagents. Anal, 1989, 114, 631-633.
- Orsonneau J.L., Douet P., Massubre C., Lustenberger P., Bernard S. An improved pyrogallol red-molybdate method for determining total urine protein. Clin Chem, 1989, 35(11), 2233-2236.
- Salem E.M., Awlya O.F.A. Substituting of cacao by carob pod powder in milk chocolate manufacturing. Aust J Basic & Appl Sci, 2012, 6(3), 572-578.
- Avallone R., Plessi M., Baraldi M., Monzani A. Determination of chemical composition of carob (Ceratoniasiliqua) Protein, Fat, carbohydrates, and Tannins. J Food Compos Anal, 1997, 10, 166-172.
- Poppe J., Gelatin in, Imeson A. (Ed.). Thickening and Gelling Agents for Food. 2nd ed. Blackie Academic and Prof, London, 1997, 144-168.
- Harbertson J.F., A Guide to the fining of wine WSU EXTENSION MANUAL EM016 (Subject code 233). Washington State University, 2009, 4.
- Bowyer P.K. Molecular polarity– it`s behind more than you think. Aust Grape Grower and Winemaker, November, 2003, 67-70.
- Guerra B. Choosing a fining agent. Wine Business Mon, Agust 15, 2009.
- Burns R.E. Method for estimation of tannin in grain sorghum. Agronomy J, 1971, 63, 511-512.
- Torah-diaz I., Jain V.K., Baratti J. Effect of fructose concentration and aeration on ethanol production by Zymomonas mobilis. Biotechnol Lett, 1983, 5(10), 697-702.
- Onsoy T., Thanonkeo P., Thanonkeo S., Yamada M. Ethanol production from Jerusalem artichoke by Zymomonas mobilis in batch fermentation. KMITL Sci Technol J (King Mongkut's Institute of Technol Ladkrabang Thailand), 2007, 7(S1), 55–6.
- Mullins J.T., Lee J.H. Interactions of tannins with enzymes: A potential role in the reduced rate of ethanol fermentation from high-tannin biomass. Biomass Bioenerg, 1991, 1(6), 355-361.
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