Document Type : Research Article


1 Faculty of Agriculture and Natural Resources, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran

2 Water Research Center, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran


The anaerobic digestion of organic waste for biogas production can be affected by some variables such as temperature; concentration of the biogas feed solution, bacteria populations, and pressure. This study investigated the effects of thermal pretreatment at 50, 75, and 100 ºC on the biogas produced by simultaneous anaerobic digestion of cow manure, mushroom waste, and wheat straw at thermophilic temperature. Moreover, the effects of a zeolite on reducing the salinity of the wastewater were evaluated. Cow manure, mushroom waste, and wheat straw were mixed to yield a mixture with an optimum carbon to nitrogen ratio of 20-30 and TS of 25-35%. Each thermal pretreatment was prepared in four replicates and placed in a steam bath with a temperature of 55 ºC. The amount of gas produced by each thermal pretreatment was measured every day for 15 days. On day 15, the electrical conductivity of the produced wastewater was measured and the wastewater was exposed to a modified zeolite. The results showed that the greatest level of biogas was produced by thermal pretreatment at 75 ºC, which gave the biogas yield of 0.197 L/gVS after 15 days observation while, the other thermal pretreatments at 50, and 100 ºC gave the biogas yield 0.147, and 0.169 L/gVS, respectively. The highest amount of biogas was achieved on the third day for every three thermal pretreatments. Moreover, the modified zeolite reduced the wastewater salinity by 25%. These results confirmed that thermal pretreatment at 75 ºC is an effective pretreatment for biogas production improvement from the mixture of cow manure, mushroom waste, and wheat straw, and the modified zeolite could be used for salinity reduction of wastewater discharged from the process.


1.     Hung, C. and Solli, C., "Biogas from municiapal organic waste–trondheim's environmental holy grail?", Energy Procedia, Vol. 20, (2012), 11-19.
2.     Hendroko, S.R., Wahono, S.K., Praptiningsih, G.A., Yudhanto, A.S., Wahyudi, I. and Dohong, S., "The study of optimization hydrolysis substrate retention time and augmentation as an effort to increasing biogas productivity from Jatropha curcas Linn. Capsule husk at two stage digestion", Energy Procedia, Vol. 47, (2014), 255-262.
3.     Sitorus, B. and Panjaitan, S.D., "Biogas recovery from anaerobic digestion process of mixed fruit-vegetable wastes", Energy Procedia, Vol. 32, (2013),176-182.
4.     Sharifi, M.A., Haghighi, M., Rahmani, F. and Rahemi, N., "Reforming of biogas over Co-and Cu-promoted Ni/Al2O3-ZrO2 nanocatalysts synthesized via sequential impregnation method", Journal of Renewable Energy and Environment, Vol. 1, No. 1, (2014), 53-63.
5.     Nosratinia, M., Tofigh, A.A. and Adl. M., "Making decision support system for utilization of biogas in Iran", Journal of Renewable Energy and Environment, Vol. 2, No. 1, (2014), 1-5.
6.     Mel, M., Ihsan, S.I. and Setyobudi, R.H., "Process improvement of biogas production from anaerobic co-digestion of cow dung and corn husk", Procedia Chemistry, Vol. 14, (2015), 91-100.
7.     Samani, S., Abdoli, M.A., Karbassi, A. and Amin, M.M., "Stimulation of the hydrolytic stage for biogas production from cattle manure in an electrochemical bioreactor", Water Science and Technology, Vol. 74, No. 3, (2016), 606-615.
8.     Samani Majd, S., Abdoli, M.A., Karbassi, A., Pourzamani, H.R. and Rezaee, M., "Effect of Physical and Chemical Operating Parameters on Anaerobic Digestion of Manure and Biogas Production: A Review", Journal of Environmental Health and Sustainable Development, Vol. 2, No. 1, (2017), 235-247.
9.     Karlsson, N.P., Halila, F., Mattsson, M. and Hoveskog, M., "Success factors for agricultural biogas production in Sweden: A case study of business model innovation", Journal of Cleaner Production, Vol. 142, (2017), 2925-2934.
10.   Al-Masri, M.R., "Changes in biogas production due to different ratios of some animal and agricultural   wastes", Bioresource Technology, Vol. 77, No. 1, (2001), 97-100.
11.   Carrere, H., Antonopoulou, G., Affes, R., Passos, F., Battimelli, A., Lyberatos, G. and Ferrer, I., "Review of feedstock pretreatment strategies for improved anaerobic digestion: from lab-scale research to full-scale application", Bioresource Technology, Vol. 199, (2016), 386-397
12.   Ferreira, L.C., Souza, T.S.O., Fdz-Polanco, F. and Pérez-Elvira, S.I., "Thermal steam explosion pretreatment to enhance anaerobic biodegradability of the solid fraction of pig manure", Bioresource Technology, Vol.152, (2014), 393-398.
13.   Neumann, P., Pesante, S., Venegas, M. and Vidal, G., "Developments in pre-treatment methods to improve anaerobic digestion of sewage sludge", Reviews in Environmental Science and Bio/Technology, Vol. 15, No. 1, (2016), 173-211.
14.   Ferreira, L.C., Donoso-Bravo, A., Nilsen, P.J., Fdz-Polanco, F. and Pérez-Elvira, S.I., "Influence of thermal pretreatment on the biochemical methane potential of wheat straw", Bioresource Technology, Vol. 143, (2013), 251–257.
15.   Qiao, W., Yan, X., Ye, J., Sun, Y., Wang, W. and Zhang, Z., "Evaluation of biogas production from different biomass wastes with/without hydrothermal pretreatment", Renewable Energy, Vol. 36, (2011), 3313–3318.
16.   Ardic, I. and Taner, F., "Effects of thermal, chemical and thermochemical pretreatments to increase biogas production yield of chicken manure", Fresenius Environment Bulletin, Vol. 14, (2005), 1–8.
17.   Bujoczek, G., Oleszkiewicz, J., Sparling, R. and Cenkowskic, S., "High Solid Anaerobic Digestion of Chicken Manur", Journal of Agricultural Engineering Research, Vol. 76, (2000), 51–60.
18.   Chae, K.J., Jang, A., Yim, S.K. and Kim, S., "The effects of digestion temperature and temperature shock on the biogas yields from the mesophilic anaerobic digestion of swine manure", Bioresource Technology, Vol. 99, (2008), 1–6.
19.   Mladenovska, Z., Hartman, H., Kvist, T., Sales-Cruz, M., Gani, R. and Ahring, B.K., "Thermal pretreatment of the solid fraction of manure: impact on the biogas reactor performance and microbial community", Water Science Technology, Vol. 53,  No. 8, (2006), 59–67.
20.   Menardo, S., Balsari, P., Dinuccio, E. and Gioelli, F., "Thermal pre-treatment of solid fraction from mechanically-separated raw and digested slurry to increase methane yield", Bioresource Technology, Vol. 102, (2011), 2026–2032.
21.   Rau, I., Gonzalo, A. and Valiente, M., "Arsenic (V) adsorption by immobilized iron mediation. Modeling of the adsorption process and influence of interfering anions", Reactive and Functional Polymers, Vol. 54, No. 1, (2003), 85-94.
22.   Borghei, M., Lahijani M., Hosni A., Salari M. and Sayyadi M., "Evaluation of the function of natural zeolites in reducing the salinity of Groundwater", Sharif Civil Engineering Journal, Vol. 3, (2014), 97-105.
23.   Egashira, R., Tanabe, S. and Habaki, H., "Adsorption of heavy metals in mine wastewater by Mongolian natural zeolite", 20th International Congress of Chemical and Process Engineering CHISA 2012, (2012), 25– 29, 49-57.
24.   Shukla, E., johan, E., Henmi, T. and Matsue, N., "Arsenate adsorption on iron modified artificial zeolite made from coal fly ash", Procedia Environmental Sciences, Vol. 17, (2013), 279 – 284.
25.   Russo, A. and Jacobo, E., "Removal of MTBE in Columns filled with Modified Natural Zeolites", Procedia Materials Science, Vol. 8, (2015), 375 – 382.
26.   2Andrew, D.E., "Standard Methods for the Examination of Water and Wastewater, American Public Health Association (APHA)", 21 st ed, Washington, D.C.: APHA-AWWA-WEF, (2005).
27.   Dioha, I.J., Ikeme, C.H., Nafi’u, T., Soba, N.I. and Yusuf, M.B.S., "Effect of carbon to nitrogen ratio on biogas production", International Research Journal of Natural Sciences, Vol. 1, No. 3, (2013), 1-10..
28.   Chao, C.C. and Rastelli H., "Process for modifying clinoptilolite adsorbent", U.S. Patent No, 5,116,793, Washington, DC: U.S, Patent and Trademark Office, (1992).
29.   Taherdanak, M. and Zilouei, H., "Improving biogas production from wheat plant using alkaline pretreatment", Fuel, Vol. 115, (2014), 714-719.
30.   Rafique, R., Poulsen, T.G., Nizami, A.S., Murphy, J.D. and Kiely, G., "Effect of thermal, chemical and thermo-chemical pre-treatments to enhance methane production", Energy, Vol. 35, No. 12, (2010),  4556-4561.
31.   Carrère, H., Sialve, B. and Bernet, N., "Improving pig manure conversion into biogas by thermal and thermo-chemical pretreatments", Bioresource Technology, Vol. 100, No. 15, (2009), 3690-3694.
32.   González-Fernández, C., Sialve, B., Bernet, N. and Steyer, J.P., "Thermal pretreatment to improve methane production of Scenedesmus biomass", Biomass and bioenergy, Vol. 40, (2012), 105-111.
33.   Sand, L.B. and Mumpton, F.A., "Natural zeolites: occurrence, properties, and use (No. CONF-760626-(Exc.))", Pergamon Press, Inc, Elmsford, NY, (1978).
34.   34.  Wang, S. and Peng, Y., "Natural zeolites as effective adsorbents in water and wastewater treatment ", Chemical Engineering Journal, Vol. 156, No. 1, (2010), 11-24.
35.   Wajima, T., "Ion exchange properties of Japanese natural zeolites in seawater", Analytical   Sciences, Vol. 29, No. 1, (2013), 139-141.
36.   Liu, F., Ma, B.R., Zhou, D., Xiang, Y.H. and Xue, L.X., "Breaking through tradeoff of   Polysulfone ultrafiltration membranes by zeolite 4A", Microporous and Mesoporous Materials, Vol. 186, (2014), 113-120.
37.   Shekarian, F., Soleimani, K., Nematzadeh, G.H. and Biparva, P., "Evaluation of equilibrium isotherms of zeolite and perlite in adsorption Sodium chloride salt from aqueous solution", Journal of Ecohydrology, Vol. 3, (2014), 223-232.